| CAS-Number: | 27072-45-3 |
|---|---|
| Molecular Weight: | 389.38 |
| Empirical Formula: | C21H11NO5S |
| Absorption [nm]: | 494 ± 3 |
| Emission [nm]: | 518 ± 3 |
| ε [M-1 cm-1]: | >70 000 |
| Buffer System for Fluorescence Readings: | T&S Buffer, pH 9 |
| Solubility: | DMF |
| Appearance (colour): | orange |
| Appearance (form): | solid (powder) |
| Storage: | - 20 °C. Keep dry! Keep away from light, especially in solution! |
| Trade name: | 5/6 FITC, mixed isomers |
| Reactive Moiety: | Isothiocyanate, reacts with 1° amines to form a stable thiourea linkage |
This classic amine-reactive fluorescein derivatization reagent is widely used for covalent labeling of proteins and alkylamino modified nucleic acids.
Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.
5/6-FITC
Hazard Classifications
Respiratory/skin sensitisation, Category 1 (Resp. Sens. 1)
Hazard Statements
| H334 | May cause allergy or asthma symptoms or breathing difficulties if inhaled. |
Precautionairy Statements
| P261 | Avoid breathing dust/fume/gas/mist/vapours/spray. |
| P285 | In case of inadequate ventilation wear respiratory protection. |
| P342+P311 | If experiencing respiratory symptoms: Call a POISON CENTER/doctor/… |
Signal Word
Danger
WGK
WGK 3
Storage Class Code
11 - Flammable solids
ADR, RID (road and railroad)
Not dangerous goods
IATA-DGR (transport by air)
Not dangerous goods
IMDG-Code (transport by sea)
Not dangerous goods
Gefahreneinstufungen
Sensibilisierung der Atemwege/Haut, Kategorie 1 (Resp. Sens. 1)
Gefahrenhinweise - H-Sätze
| H334 | Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen. |
Sicherheitshinweise - P-Sätze
| P261 | Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden. |
| P285 | Bei unzureichender Belüftung Atemschutz tragen. |
| P342+P311 | Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM/Arzt/… anrufen. |
Signalwort
Gefahr
WGK
WGK 3
Lagerklasse
11 - Entzündbare Feststoffe
ADR, RID (Strasse und Eisenbahn)
Kein Gefahrgut
IATA-DGR (Luftverkehr)
Kein Gefahrgut
IMDG (Seeschiffverkehr)
Kein Gefahrgut