| CAS-Number: | 75350-46-8 |
|---|---|
| Molecular Weight: | 427.4 |
| Empirical Formula: | C24H13NO7 |
| Absorption [nm]: | 492 ± 3 |
| Emission [nm]: | 513 ± 3 |
| ε [M-1 cm-1]: | >80 000 |
| Buffer System for Fluorescence Readings: | T&S Buffer, pH 9 |
| Solubility: | DMF |
| Appearance (colour): | yellow orange |
| Appearance (form): | solid (powder) |
| Storage: | - 20 °C. Keep dry! Keep away from light, especially in solution! |
| Name: | 5-(2,5-Dioxo-2,5-dihydro-pyrrol-1-yl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-benzoic acid |
| Trade name: | Fluorescein-5-maleimide, 5-FMI |
| Reactive Moiety: | Maleinimid, reacts with thiol to form a stable thioether linkage |
Readily reacts with the thiol group found on cysteine or with thiol-modified oligonucleotides to form a stable carbon-sulfur bond.