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Cleavage of the oligonucleotide from its solid support and subsequent removal of all protecting groups from the nucleobases and phosphates close the cycle of automated oligonucleotide synthesis and bring it to completion. For this purpose, three different Cleavage solutions from emp BIOTECH are available. The correct choice will depend on your requirements for standard, fast or mild cleavage conditions.
Standard: Usage of one volume of conc. ammonium hydroxide (≥ 28 %) under sealed conditions appropriate for removal of the protecting groups of the nucleobases Fast: Usage of one volume of AMA under sealed conditions at 65 °C for 10 min Mild: Usage of the required volume of Deprotection, Ultramild under sealed conditions for 8 h at room temperature
Hazard Classifications
Flammable liquid, Category 2 (Flam. Liq. 2)
Acute Toxicity, Category 4 (Acute Tox. 4)
Skin Corr. 1B
Specific target organ toxicity - single exposure, Category 3 (STOT SE 3)
Hazardous to the aquatic environment, Category 1 (Aquatic Chronic 1)
Hazard Statements
| H225 | Highly flammable liquid and vapour. |
| H302 | Harmful if swallowed. |
| H314 | Causes severe skin burns and eye damage. |
| H335 | May cause respiratory irritation. |
| H410 | Very toxic to aquatic life with long lasting effects. |
Precautionairy Statements
| P210 | Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. |
| P261 | Avoid breathing dust/fume/gas/mist/vapours/spray. |
| P280 | Wear protective gloves/protective clothing/eye protection/face protection. |
| P301+P312 | IF SWALLOWED: Call a POISON CENTER/doctor/… if you feel unwell. |
| P303+P361+P353 | IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water [or shower]. |
| P305+P351+P338 | IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
Signal Word
Danger
WGK
WGK 2
Storage Class Code
8 A - Flammable corrosive materials
ADR, RID (road and railroad)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Aqueous solution of ammonia and methylamine), 3(8), II
IATA-DGR (transport by air)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Aqueous solution of ammonia and methylamine), 3(8), II
IMDG-Code (transport by sea)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Aqueous solution of ammonia and methylamine), 3(8), II
Gefahreneinstufungen
Entzündbare Flüssigkeiten, Kategorie 2 (Flam. Liq. 2)
Akute Toxizität, Kategorie 4 (Acute Tox. 4)
Skin Corr. 1B
Spezifische Zielorgan-Toxizität (einmalige Exposition), Kategorie 3 (STOT SE 3)
Gewässergefährdend, Kategorie 1 (Aquatic Chronic 1)
Gefahrenhinweise - H-Sätze
| H225 | Flüssigkeit und Dampf leicht entzündbar. |
| H302 | Gesundheitsschädlich bei Verschlucken. |
| H314 | Verursacht schwere Verätzungen der Haut und schwere Augenschäden. |
| H335 | Kann die Atemwege reizen. |
| H410 | Sehr giftig für Wasserorganismen, mit langfristiger Wirkung. |
Sicherheitshinweise - P-Sätze
| P210 | Von Hitze, heißen Oberflächen, Funken, offenen Flammen und anderen Zündquellen fernhalten. Nicht rauchen. |
| P261 | Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden. |
| P280 | Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz/Gehörschutz/… tragen. |
| P301+P312 | BEI VERSCHLUCKEN: Bei Unwohlsein GIFTINFORMATIONSZENTRUM/Arzt/ … anrufen. |
| P303+P361+P353 | BEI BERÜHRUNG MIT DER HAUT (oder dem Haar): Alle kontaminierten Kleidungsstücke sofort ausziehen. Haut mit Wasser abwaschen [oder duschen]. |
| P305+P351+P338 | BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Eventuell vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen. |
Signalwort
Gefahr
WGK
WGK 2
Lagerklasse
8 A - Brennbare ätzende Stoffe
ADR, RID (Strasse und Eisenbahn)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Aqueous solution of ammonia and methylamine), 3(8), II
IATA-DGR (Luftverkehr)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Aqueous solution of ammonia and methylamine), 3(8), II
IMDG (Seeschiffverkehr)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Aqueous solution of ammonia and methylamine), 3(8), II