ZetaSep Desalt

ZetaSep FPLC Desalting Columns are designed for:

• Separating larger biomolecules (i.e. proteins such as antibodies, enzymes or larger nucleic acids) from unwanted smaller molecules.
• Buffer exchange, desalting, removal of low molecular weight contaminants, and reaction terminations.
• Simple, rapid and reproducible separation using a syringe, pump or liquid chromatography system.

The fractionation range for globular proteins is between 1 and 5 kD.  The size exclusion cut-off is approximately 5 kD, which ensures efficient separation of proteins/peptides/biomolecules larger than 5 kD from lower molecular weight molecules of less than 1 kD.

ZetaSep Desalting FPLC Columns contain Zetadex-25 Superfine, a beaded composite size-exclusion matrix manufactured by emp Biotech in Berlin-Buch. It exhibits high flow rates, excellent resolution and chemical stability.  Buffer and pH effects on resolution are minimal.