13th to 15th November

emp BIOTECH Delegation Attending Medica 2023.
Are you attending this year’s Medica in Düsseldorf?
More

Take this opportunity to meet with delegates from emp Biotech. Three delegates from emp Biotech will be attending Medica between Monday 13th to Wednesday 15th November. Present from emp BIOTECH will be:
• Derek Levison, Managing Director
• Alistair Hurst, Business Development Manager
• Franziska Meier-Hättig, Product Manager
Contact us if you wish to meet with the emp BIOTECH team during Medica.

Optimizing Use of Your Desalting Column

Optimizing Use of
Your Desalting Column

Desalting columns are an essential tool in the field of molecular biology, biochemistry, and protein research. They facilitate the removal of unwanted salts, small molecules, and other contaminants from a sample, purifying and concentrating target molecules in the process.

In this article, we will delve into the importance of optimizing desalting column for effective purification of samples. Desalting columns play a vital role in removing salts and other impurities, ensuring the delivery of high-quality samples for downstream applications.

Optimizing Desalting Column:
Factors to Consider

The following factors should be considered to optimize the
performance of your desalting column:

  1. Sample Preparation: Prior to using the desalting column, ensure that your sample is prepared correctly. This includes selecting the appropriate buffer, pH, and sample concentration. The buffer should be compatible with both the target molecule and the desalting column matrix. Additionally, the pH should be maintained within the optimal range for the target molecule’s stability.
  2. Column Selection: Choose the right desalting column for your specific application. EMP Biotech offers desalting columns covering a wide range of sample volumes. Selecting the appropriate column ensures optimal workflow, purification, and recovery of your target molecule.
  3. Equilibration: Before loading your sample onto the desalting column, it is essential to equilibrate the column with the desired buffer. Proper equilibration removes any preservatives and ensures that the column matrix is in the optimal condition for sample separation. Follow the manufacturer’s guidelines for equilibrating your EMP Biotech desalting column.
  4. Sample Loading: Load your sample onto the desalting column according to the manufacturer’s instructions. Be mindful of the sample volume, as overloading the column can lead to inadequate separation and reduced recovery of the target molecule. For optimal results, ensure that the sample volume does not exceed the recommended loading capacity for your specific desalting column.
  5. Elution: Elute your sample from the desalting column following the manufacturer’s recommended elution protocol. Proper elution techniques ensure the efficient recovery of your target molecule and minimize the presence of contaminants. Be sure to collect the eluted fractions in appropriate collection tubes or vessels to prevent cross-contamination.
  6. Monitoring and Evaluation: Monitor the elution process by measuring the absorbance of the collected fractions at an appropriate wavelength. This will help you track the elution profile of your target molecule and identify the fractions containing the purified molecule. Evaluate the performance of your desalting column by assessing the purity and recovery of the target molecule in the eluted fractions.

Optimizing Desalting Column:
Tips and Best Practices

To further enhance the performance and efficiency of your desalting column, consider the following tips and best practices:

    1. Maintain optimal storage conditions: Store your desalting column according to the manufacturer’s recommendations. This will help preserve the column’s performance and extend its lifespan.
    2. Clean the column, if necessary: If the desalting column’s performance declines, consider cleaning the column following the manufacturer’s guidelines. Proper cleaning can restore the column’s performance and prolong its life.
    3. Monitor the column’s performance over time: Regularly assess the performance of your desalting column by evaluating the purity and recovery of your target molecule. This will help you identify any potential issues and take appropriate action to maintain optimal column performance.

Conclusion

emp BIOTECH desalting columns are valuable tools for the purification and concentration of target molecules in various research applications.

By considering factors such as sample preparation, column selection, equilibration, sample loading, elution, and monitoring, you can optimize the use of your desalting column and achieve consistent, high-quality results. Incorporating tips and best practices such as proper storage, column cleaning, buffer optimization, and gradient elution can further enhance the performance and efficiency of your desalting column, ensuring the success of your biomolecule purification.

Are you purifying proteins or DNA?

Are you purifying proteins or DNA?

Read emp BIOTECH’s latest brochure
on Biomolecule Purification

If you already know emp BIOTECH’s desalting columns, or their SMART Chromatography™ technology, you might not be aware that emp also manufactures a range of resins, available in bulk quantities, that can be applied to a number of purification challenges.

The latest catalogue covers emp’s range in:

  • Affinity chromatography
  • Ion exchange (IEX) purification
  • Hydrophobic interaction chromatography (HIC)
  • Activated resins – for attaching your “favourite” molecules

…and of course, emp BIOTECH’s SEC columns, resins etc. are also covered.

Download:

Biomolecule Purification, Catalog 09/2023

New: TurboTag™ Protein/Antibody Labeling Kits

New: TurboTag™ Protein/Antibody Labeling Kits

emp BIOTECH’s popular protein labelling kits have
now been renamed as TurboTag™ precision protein labeling kits.

The kits will now only be available in a 3-reaction size. Everything else remains the same:

  • 10 minute labelling protocol, from start to finish
  • Optimized protocols for 1 mg and 100 µg of protein
  • Over 35 different fluorescent and non-fluorescent labels to choose from

Download:

TurboTag™ Labeling Kits, Flyer 09/2021


Find all products:

Search by TurboTag

Watch our video to get an overview
of TurboTag™ technology.

Open the box, label the protein and
purify – all in 10 minutes.